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EMBRYOLOGY

GEORGE H. SHULL.

On the Totipotence of the First Two Blastomeres of the Frog's Egg. It was found by Roux that if one of the first two blastomeres of the frog's egg is injured with a hot needle, the uninjured blastomere will develop into a half embryo. Morgan found, however, that if the egg were kept in an inverted position after the injury of one blastomere, the other would develop into a whole embryo. He attributes this difference to the rearrangement of the "mosaic" structure of the egg by flowing of substance, of different specific gravity (Born). However, he suggested to me that the half embryo obtained by Roux might be due to the presence of the injured blastomere. In Triton, when the first two blastomeres are separated, each gives rise to a whole embryo (in case the first cleavage plane would have become the median plane). I have been trying various methods for removing one of the first two blastomeres of the frog's egg, and succeeded in getting a small per cent. of the remaining blastomeres (of Chorophilus triseriatus) to develop. The puncture of the egg membrane caused it to shrink down on the remaining blastomere and left an opening for bacteria. Although there was a great mortality in the operated eggs, quite a number of them gastrulated (as wholes), and several of them reached the tadpole stage. The pressure of the egg membrane seemed to hinder their further development and none of them hatched, although one lived until I fixed it for sectioning, after the control eggs had hatched. In no case was a half gastrula found, and as this is the earliest stage at which the bilateral symmetry is very pronounced, all of the embryos were wholes as far as could be observed. Probably all operated eggs in which the median plane would not have coincided with the first cleavage plane died before gastrulation.

UNIVERSITY OF MISSOURI, April 8, 1909.

J. F. MCCLENDON.

A Monthly Journal, established in 1867, Devoted to the Advancement of the Biological Sciences with Special Reference to the Factors of Organic Evolution and Heredity

CONTENTS OF THE DECEMBER NUMBER Some Physiological Aspects of Radium Rays. Professor C. STUART GAGER.

On the Origin of Structures in Plants. W. A. CANNON. Origin and Formation of the Froth in Spittle Insects, BRAXTON H. GUILBEAU.

Shorter Articles and Correspondence: Peculiar Abnor mal Teeth in a Jack Rabbit. WILLIAM A. HILTON, Notes and Literature: Ichthyology- Ichthyological

Notes, President DAVID STARR JORDAN, The Inheritance of Sex in Higher Plants-Digest of Professor C. Correns's Memoir: Professor H. E. JORDAN, Title Page and Index to Volume XLII.

CONTENTS OF THE JANUARY NUMBER Juvenile Kelps and the Recapitulation Theory. Pro fessor ROBERT F. GRIGGS,

The Larva and Spat of the Canadian Oyster, Dr. J. STAFFORD.

Shorter Articles and Correspondence: Some Notes on the Traditions of the Natives of Northeastern Siberia about the Mammoth, WALDEMAR JOCHELSON. Age of Trotting Horse Sires, F. R. MARSHALL, The Occurrence of Batrachoceps attenuatus and Autodaz lugubris in Southern California, WILLIAM A. HILTON.

Notes and Literature: Experimental Evolution-The Effect of the Environment upon Animals, Dr. FRANK E LUTZ. Experimental Zoology-The Influence of the Size of the Egg and Temperature on the Growth of the Frog, SERGIUS MORGULIS. Parasitology-Cestodes of Birds, Professor HENRY B.

WARD.

CONTENTS OF THE FEBRUARY NUMBER Charles Darwin and the Mutation Theory. CHARLES F. Cox.

Juvenile Kelps and the Recapitulation Theory. II. Professor ROBERT F. GRIGGS.

Notes and Literature: Plant Phylogeny-The Origin of the Archegoniates, Dr. BRADLEY M. DAVIS. Holothurians-Clark's The Apodous Holothurians, W. K FISHER. Lepidoptera-The Blue Butterflies of the Genus Celastrina, Professor T. D. A. COCKERELL. Vertebrate Paleontology-The Lysorophida; Stegocephala; The Cotylosauria; The Oldest Known Reptile; The Age of the Gaskohle; Bison Occidentalis; Nectosaurus; Callibrachion, Dr. Roy L. MOODIE, Parasitology-The Sleeping Sickness Bureau, Professor HENRY B. WARD. Exploration-Camp-fires on Desert and Lava, Dr. Roy L. MOODIE,

CONTENTS OF MARCH NUMBER Invitation Papers at the Baltimore Meeting of the Botanical Society of America; Darwin Memorial Session:--Darwin as a Naturalist; Darwin's Work on Cross Pollination in Plants. Professor WILLIAM TRELEASE.

Darwin's Influence upon Plant Geography and Ecol-
ogy. Professor FREDERIC E. CLEMENTS.
Darwin's Work on Movement in Plants. Professor
HERBERT MAULE RICHARDS.

An Examination of Darwin's "Origin of Species" in the
Light of Recent Observations and Experiments.
Professor EDWIN LINTON.

The Distinction between Development and Heredity in Inbreeding. Dr. EDWARD M. EAST.

Breeding Experiments with Rats. Professor T. H.
MORGAN.

Shorter Articles and Discussion: The Chub and the Texas
Horn Fly, Dr. ROY L. MOODIE, A New Camel from
the Lower Miocene of Nebraska, HAROLD JAMES
Cook
Notes and Literature: Heredity-The Chondriosomes as
Bearers of the Hereditary Qualities, F. PAYNE,
Cultural Bed Mutations in the Potato.

CONTENTS OF THE APRIL NUMBER Heredity of Hair Color in Man, GERTRUDE C. DAVENPORT and CHARLES C. DAVENPORT.

A Mechanism for Organic Correlation. Professor G, H. PARKER.

Recent Advances in the Study of Vascular Anatomy.

Vascular Anatomy and the Reproductive Structures.
Professor JOHN M. COULTER.

The Progress of Plant Anatomy During the Last
Decade. Professor EDWARD C. JEFFREY.
Shorter Articles and Correspondence: A Note on the
Degree of Accuracy of the Biometric Constants, DR.
RAYMOND PEARL Pure Strains as Artifacts of
Breeding. O. F. COOK.

Notes and Literature: Heredity-The Nature of "Unit"
Characters, DR. W. J. SPILLMAN. Environment—
DR. FRANK E LUTZ. Experimental Zoology-Hy-
bridology and Gynandromorphism, Professor T. H.
MORGAN.
Echinodermata-Red Sea Crinoids, Dr.

AUSTIN HOBART CLARK,

CONTENTS OF THE MAY NUMBER

The Categories of Variation. Professor S. J. HOLMES. The General Entomological Ecology of the Indian Corn Plant: S. A. FORBES.

Notes and Literature: Biometrics-Some Recent Studies on Growth. DR. RAYMOND PEARL. Experimental Zoology-Cuénot on the Honey Bee, Professor T. H. MORGAN. The Upholding of Darwin-Poulton and Plate on Evolution, V. L. K.

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