Lipid Analysis: Isolation, Separation, Identification, and Structural Analysis of Lipids
Paperback. This book is a practical guide in one compact volume to the complexities of the analysis of lipids from animal and plant tissues. Methods for chromatography, chemical analysis and spectroscopy of lipids are critically described with 900 references. The basic principles of each technique are discussed and selected procedures are described in such detail that the book should serve as a primary source, of value at the laboratory bench. Chapters follow a logical sequence from the extraction of lipids from tissues to the isolation and characteriaation of particular lipid classes and of the molecular species of each. In this second edition there is a chapter on the separation of lipoprotein classes.
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The Structure Chemistry and Occurrence of Lipids
Chromatographic and Spectroscopic Analysis of Lipids General Principles
The Isolation of Lipids from Tissues
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adsorbent adsorption aldehydes alkyl amounts analysis animal tissues aqueous Biochem cent ceramide chain chain-length Chapter chemical chloride chloroform chloroform-methanol cholesterol esters Chromat column chromatography complex lipids components compounds containing derivatives described detected detector determined diacylglycerol acetates diethyl ether double bonds EGSS-X eluted enzyme esterified extracted fatty acid composition fractions free fatty acids functional groups gangliosides gas chromatography gel G glycerides glycerol glycolipids hexane hexane-diethyl ether HPLC hydrogen hydrolysis isolated isomers layers of silica lipid classes lipids lipoproteins liquid phases long-chain bases methanol method methyl methyl esters migration mixture moieties molecular species molecule monoenoic necessary obtained pancreatic lipase peaks phosphatidic acid phosphatidylcholine phospholipids plasmalogens polar polyunsaturated fatty acids position prepared procedure radioactivity reaction reagent removed sample saturated scintillation separated silica gel silicic acid silver nitrate simple lipids sodium solution solvent solvent system spray stationary phase technique temperature thin-layer TLC plates transesterified triacylglycerols unsaturated washed