Lipid Analysis: Isolation, Separation, Identification, and Structural Analysis of Lipids
Paperback. This book is a practical guide in one compact volume to the complexities of the analysis of lipids from animal and plant tissues. Methods for chromatography, chemical analysis and spectroscopy of lipids are critically described with 900 references. The basic principles of each technique are discussed and selected procedures are described in such detail that the book should serve as a primary source, of value at the laboratory bench. Chapters follow a logical sequence from the extraction of lipids from tissues to the isolation and characteriaation of particular lipid classes and of the molecular species of each. In this second edition there is a chapter on the separation of lipoprotein classes.
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The Isolation of Lipids from Tissues
Chromatographic and Spectroscopic Analysis of Lipids General Principles
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acetic added addition adsorbent amounts analysis animal appear applied band bases carbon cent Chapter chemical chloroform cholesterol column complex lipids components composition compounds containing derivatives described detected determined developed diethyl ether discussed double bonds effect eluted estimated ether example extracted fatty acids fractions further gas chromatography give glycerol glycolipids groups hydrolysis identified isolated layers less mass material means methanol method methyl esters migrate mixture moieties molecular species natural necessary obtained occur original packed particular peaks phase phospholipids plant plate polar polyunsaturated position possible prepared present procedure reaction reagent recovered removed resolution reviewed sample saturated separated silica gel silver nitrate similar simple lipids single sodium solution solvent solvent system species spray standard stationary phase structural suitable technique temperature tissues trace triacylglycerols unsaturated usually various volumes washed weight